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Methods Cell culture Human lung adenocarcinoma cell lines (A549, H322, H1299, H1792, H1975, H2009, PC9) were previously obtained 87 from the Harmon Cancer Center Collection (University of Texas-Southwestern Medical Center) or commercially from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium (Fisher Scientific, 11-875-119) supplemented with 5% FBS (Sigma-Aldrich, F0926) in the absence of antibiotics

J Appl Physiol 8: 1118 Borbely AA (1982) A two-process model of sleep regulation

Glass bottom dishes were used for cell culture due to confocal scanning requirements

The tissue sections of plerocercoids and adult worms was first retrieved after microwaving for 20 min with a 0.01 M citric acid buffer (pH 6.0), blocking with 5% normal goat serum in PBS, then incubating at 37C or 1 h with a 1: 10 dilution of anti-rSmGST serum, serum of mice infected with plerocercoids, and normal mouse serum or PBS, respectively