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This suggesting that activation of the renal IGF-1 signaling may not be directly involved in the GH-induced pathological effects on the kidney.10,11 The mild glomerular changes observed, such as cell proliferation, could be mediated directly by renal IGF-1, since mesangial and other glomerular cells express the IGF1R.12 Interestingly, other studies have observed increased kidney weight following IGF-1 or GH administration in rats

An adequate volume of each cell culture was inoculated into 20 mL of the same medium in 200 mL of baffled erlenmeyer flask with or without 300 mg/L l-Ser to achieve an initial OD 600 value of 0.15

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